Protein quantification analysis-Label-free
Biotools also provide you the label-free analysis to quantify the protein between different samples by using PSM (peptide spectrum match).
You could use the label-free analysis to screen the potential biomarker, then for the more accurate result, you could choose the i-TRAQ analysis.
You could use the label-free analysis to screen the potential biomarker, then for the more accurate result, you could choose the i-TRAQ analysis.
Without the stable isotope labeling, biotools provide you label-free analysis for protein quantification by comparing the peptide spectrum match (PSM) between different samples.
The sample number will not be limited by this method, you could use this method to screen the potential biomarker.
We will recommend you after the label-free analysis, you could use i-TRAQ analysis for the more accurate result.
Service specification
The sample number will not be limited by this method, you could use this method to screen the potential biomarker.
We will recommend you after the label-free analysis, you could use i-TRAQ analysis for the more accurate result.
Service specification
| Analysis | |
| Specification | 1D, 120 min |
| Protein number can be quantified | 100-1000 |
Working day not including delivery time: 3 weeks
1. In-gel protein identification: Basic analysis
(a) Sample type: Gel slice (please provide gel image with staining)
(b) Gel size: ≤3 mm x 8 mm x 1.5 mm (Height x Width x Thickness)
(c) Platform: 1DLC, LTQ-Orbitrap MS
2. In-gel protein identification: Standard/Advance analysis
(a) Sample type: Cell pellet, Protein extracts
(b) Sample volume: ≤50 uL
(c) Sample concentration: ≥0.2 ug/uL (silver stain), ≥1 ug/uL (Coomassie blue stain)
(d) Platform: Hoefer SE260, 1DLC, LTQ-Orbitrap MS
3. In-solution protein identification: Basic/Standard/Advance analysis
(a) Sample type: Cell pellet, protein extracts (please provide buffer constituents)
(b) Sample volume: ≤100 uL
(c) Sample concentration: ≥0.1 ug/uL (Basic/Standard analysis), ≥0.5 ug/uL(Advanced analysis)
(d) Platform: 1DLC, 2DLC, LTQ-Orbitrap MS
Please click the link below for the specification of Proteomics service
Sample requirement
(a) Sample type: Gel slice (please provide gel image with staining)
(b) Gel size: ≤3 mm x 8 mm x 1.5 mm (Height x Width x Thickness)
(c) Platform: 1DLC, LTQ-Orbitrap MS
2. In-gel protein identification: Standard/Advance analysis
(a) Sample type: Cell pellet, Protein extracts
(b) Sample volume: ≤50 uL
(c) Sample concentration: ≥0.2 ug/uL (silver stain), ≥1 ug/uL (Coomassie blue stain)
(d) Platform: Hoefer SE260, 1DLC, LTQ-Orbitrap MS
3. In-solution protein identification: Basic/Standard/Advance analysis
(a) Sample type: Cell pellet, protein extracts (please provide buffer constituents)
(b) Sample volume: ≤100 uL
(c) Sample concentration: ≥0.1 ug/uL (Basic/Standard analysis), ≥0.5 ug/uL(Advanced analysis)
(d) Platform: 1DLC, 2DLC, LTQ-Orbitrap MS
Please click the link below for the specification of Proteomics service
Sample requirement
1. Summary report. pdf
2. Protein-peptide report (Identification result and Raw ratio). xlsx
3. Normalized Protein Ratio (Global normalization). xlsx
4. Differential protein list. xlsx
2. Protein-peptide report (Identification result and Raw ratio). xlsx
3. Normalized Protein Ratio (Global normalization). xlsx
4. Differential protein list. xlsx